SunTag-Based Single-Molecule Translation Imaging in <i>Caenorhabditis elegans</i>.

ABSTRACT: Translation is a key step in decoding the genetic information stored in DNA. Regulation of translation is an important step in gene expression control and is essential for healthy organismal development and behavior. Despite the importance of translation regulation, its impact and dynamics remain only partially understood. One reason is the lack of methods that enable the real-time visualization of translation in the context of multicellular organisms. To overcome this critical gap, microscopy-based methods that allow visualization of translation on single mRNAs in living cells and animals have been developed. A powerful approach is the SunTag system, which enables real-time imaging of nascent peptide synthesis with high spatial and temporal resolution. This protocol describes the implemen

SUBMITTER: van der Salm E 

PROVIDER: S-EPMC12580651 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature