Transcriptional regulation of porcine PABPN1 gene in adipogenesis.

ABSTRACT:

Objective

This study aims to reveal the transcriptional regulatory mechanism and effects of poly(A)-binding protein nuclear 1 (PABPN1) on adipogenesis, along with associated polymorphisms.

Methods

Transcription factors were identified using dual-luciferase reporter assay, overexpression techniques site-directed mutagenesis, real-time quantitative polymerase chain reaction (qPCR), electrophoretic mobility shift assays and chromatin immunoprecipitation-qPCR. Preadipocyte differentiation was measured with gain- and loss-of-function, Oil Red O staining and extraction assays. Single nucleotide polymorphisms (SNPs) were identified with direct sequencing of PCR products in the promoter, and the effects of these SNPs on PABPN1 expression were identified with dual-luciferase reporter

SUBMITTER: Zhu RR 

PROVIDER: S-EPMC12580747 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature