CTCF Is Essential for the Development and Maintenance of CALM-AF10-Induced Leukemia.

ABSTRACT: CALM (Clathrin Assembly Lymphoid Myeloid Leukemia)-AF10, a fusion gene commonly associated with acute myeloid leukemia (AML), arises from the t(10;11) translocation and is linked to poor prognosis. In this study, we demonstrate that the CCCTC-binding factor (CTCF) plays a critical role in both the initiation and maintenance of CALM-AF10-induced AML (CALM-AF10 AML). In vivo, CTCF deficiency significantly extended the survival of CALM-AF10 AML mice. In vitro, CTCF knockout (KO) reduced the colony-forming capacity of CALM-AF10 AML cells and induced their differentiation into macrophage-like cells. RNA sequencing (RNA-seq) of CTCF KO cells revealed that Transglutaminase 2 (TGM2) was the most significantly downregulated gene. Chromatin immunoprecipitation sequencing (ChIP-seq) analysis indicate

SUBMITTER: Kuroki Y 

PROVIDER: S-EPMC12580881 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature