Exosomal small non-coding RNA profiling and the role of PIWI-interacting RNA pathway genes in Lumpy skin disease virus-infected bovines.

ABSTRACT:

Objective

Lumpy skin disease (LSD) is a reemerging viral disease impacting cattle and buffaloes, posing substantial economic risks. However, the expression profile of noncoding RNAs (ncRNAs) in LSD virus (LSDV)-infected bovines has yet to be investigated. In this study, we employed small RNA sequencing (RNA-seq) to assess the expression of various ncRNAs in serum-derived exosomes from LSDV-infected bovines. We particularly focused on the bio-functional activity of PIWI-interacting RNAs (piRNAs).

Methods

Cattle were infected with a 106.5 TCID50/mL LSDV Vietnam/HaTinh/CX01 (HT10) strain and ncRNAs expression in the serum of infected cattle was analyzed small RNA-seq.

Results

We identified 426 significantly differentially expressed (DE) piRNAs in serumderived exosomes fr

SUBMITTER: Truong AD 

PROVIDER: S-EPMC12580968 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature