Mitochondrial Cell-Type-Specific Profiling: Differential Function of Mesophyll and Guard Cells is Reflected by Their Mitochondrial Proteome.

ABSTRACT: Cell specification and ensuing division of labor enable the accomplishment of complex tasks in multicellular organisms. In plants, mesophyll and guard cells exhibit striking functional, metabolic, and mechanophysical differences: mesophyll cells are highly specialized for efficient light-energy conversion, whereas guard cells regulate gas exchange. Here, we address whether the cellular specialization is sustained by mitochondrial diversity between cells. We developed mRACE (RApid CEll-type-specific isolation of mitochondria), an innovative method for rapid cell-type-specific mitochondrial isolation. mRACE involves biotin labeling of mitochondria in target cells, followed by affinity-based organelle enrichment with streptavidin-coated microbeads or immobilization on functionalized glass sli

SUBMITTER: Hater F 

PROVIDER: S-EPMC12581003 | biostudies-literature | 2025 Nov-Dec

REPOSITORIES: biostudies-literature