Probing Rigidity and Fluidity in the Interfacial Region of Lipid Bilayers with a Novel IR Probe.

ABSTRACT: We report on development of a vibrational probe (N₃-C₁₂H₂₅, az12) suitable for reporting on the rigidity and fluidity of the interfacial region in lipid bilayers. Such probe can help assess critical biological functions of cell membranes, including membrane permeability. We demonstrated a high sensitivity of the az12 probe to the rigidity of the interfacial region, manifested in the sensitivity of the width of the N₃ moiety asymmetric stretching mode absorption peak. The structural and dynamic changes associated with the gel-liquid-crystal phase transition (Lβ-Lα) for three different lipids dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylglycerol (DPPG), and egg sphingomyelin (SM) were studied using FTIR and two-dimensional infrared

SUBMITTER: Islam MM 

PROVIDER: S-EPMC12581132 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature