Label-free fluorescent probe based on upconversion nanoparticles for convenient detection of cytochrome c.

ABSTRACT: Abnormal levels of cytochrome c (Cyt c) are closely associated with various human diseases, including neurodegenerative disorders and cancers. Therefore, the quantitative detection of Cyt c holds significant value for both disease diagnosis and therapeutic efficacy assessment. Herein, we developed two novel label-free fluorescent probes for sensitive Cyt c detection based on upconversion nanoparticles: one functionalized with poly (ethylene glycol) ylated phospholipid (DSPE-PEG) alone, and the other co-functionalized DSPE-PEG combined with triphenylphosphine. The electrostatic interaction generates resulting in a quantifiable fluorescence quenching effect. The proposed method demonstrated excellent performance in the detection of Cyt c in complex matrices, including cell lysates and human

SUBMITTER: Zheng H 

PROVIDER: S-EPMC12581187 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature