CLIP2: a novel functional player in AQP5 trafficking dynamics and implications for Sjogren's disease.

ABSTRACT:

Background

Sjögren's disease (SjD) is an autoimmune condition affecting exocrine glands, resulting in decreased saliva and tear production. Aquaporin 5 (AQP5) is a transmembrane water channel playing a central role in water transport. In SjD, deregulation of AQP5 expression, trafficking and interactions with various proteins contribute to the underlying disease mechanisms.

Methods

Co-immunoprecipitation followed by mass spectrometry, proximity ligation assay, microscale thermophoresis and in-silico modelling were carried out to investigate protein-protein interaction. CRISPR/Cas9-mediated knockout was performed in a salivary gland cell line stably transfected with AQP5. Protein colocalization and AQP5 trafficking were studied by immunofluorescence. Autoantibodies were quanti

SUBMITTER: D'Agostino C 

PROVIDER: S-EPMC12581382 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature