Ultrahigh-throughput screening of heterogeneous vancomycin-intermediate Staphylococcus aureus based on fluorescence-activated droplet sorting.

ABSTRACT:

Background

Heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) is associated with suboptimal glycopeptide treatment outcomes. However, conventional antimicrobial susceptibility testing fails to distinguish hVISA from vancomycin-susceptible Staphylococcus aureus (VSSA), potentially delaying effective therapy. The gold standard population analysis profiling with area under the curve (PAP-AUC) method is labor-intensive and time-consuming, necessitating rapid detection alternatives.

Methods

This study introduces a microfluidic platform based on fluorescence-activated droplet sorting (FADS) for the rapid detection and isolation of hVISA. Through systematic optimization of key parameters such as droplet generation conditions, bacterial suspension concentration, flu

SUBMITTER: Cheng X 

PROVIDER: S-EPMC12581545 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature