Microbial Transglutaminase Increases Uptake and Translocation of Gliadin Peptides in the Human Intestinal Epithelium.

ABSTRACT: Transglutaminase 2 (TG2) plays an essential role in the pathogenesis of celiac disease (CD) by modifying gliadin peptides. Additionally, microbial transglutaminase (mTG) was implicated in CD pathogenesis since it modifies gliadin peptides in the same way. mTG is used as a technical aid in the production of food items and might also be released by the intestinal microbiota. We aimed to characterize the interaction of mTG with different gliadin peptides and its effect on the epithelial and mucosal transport.We compared mTG- and TG2-mediated crosslinking of gliadin peptides using indirect enzyme-linked immunosorbent assay (ELISA) and immunoblot. Epithelial uptake of gliadin peptides P56-88 and P31-49 by Caco-2 cells and human duodenal biopsies was quantified by fluorometry and immunofluoresce

SUBMITTER: Stricker S 

PROVIDER: S-EPMC12581733 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature