Differential Phase Contrast Imaging to Predict MSC Immune Function.

ABSTRACT: Mesenchymal stromal cells (MSCs) exhibit significant immunomodulatory potential, making them promising candidates for cell-based therapies in autoimmune and inflammatory diseases. However, the heterogeneity of MSC cultures and a lack of robust, predictive potency assays have hindered their clinical translation. In this study, the potential of single-cell morphological imaging during MSC expansion is explored as a method to estimate indoleamine-2,3-dioxygenase (IDO) protein and enzyme activity, a common immunosuppressive capacity measure. Fluorescence and label-free quantitative differential phase contrast (qDPC) imaging is employed to non-invasively extract morphological features from live MSCs during biomanufacturing with machine learning (ML) regression models to predict single cell IDO

SUBMITTER: Rui K 

PROVIDER: S-EPMC12581882 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature