Detailed morphological characterization and improvement of keratinocyte outgrowth from plucked human hair follicle.

ABSTRACT: In this study, a detailed analysis of the outgrowth of primary keratinocytes from plucked human hair follicles was conducted. Plucked hair follicles offer an easily accessible and non-invasive method as a primary cell source for adult somatic keratinocytes, providing a simple starting material for induced pluripotent stem cell (iPSC) reprogramming. In this study, we laid our focus on the precise examination of timing and location of the first keratinocyte outgrowth after plucking, as well as the morphological changes that occur during cultivation. Our results show that the region of the hair follicle, from which the initial outgrowth occurs, is crucial for successful cultivation. Additionally, first appearing protrusions and first visible cells show the same specific marker expression as t

SUBMITTER: Klingenstein S 

PROVIDER: S-EPMC12581916 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature