Kinetic measurement of gene-specific RNA polymerase II transcription elongation rates.

ABSTRACT: Transcription is regulated at multiple levels, including initiation, elongation, and termination. Whereas much research has focused on the initiation of transcription, regulation of elongation plays an important role not only in transcription dynamics but also in cotranscriptional RNA processing and genome stability. Despite advances in high-throughput approaches for global quantification of RNA polymerase II (RNAPII) speed, RNAPII elongation rate studies have been limited to a relatively small number of long genes or to velocity estimates inferred indirectly from RNAPII occupancy data. Here, we present DRB/TT_chem-seq2, a modified version of the DRB/TT_chem-seq method, to directly measure gene-specific elongation rates of more than 3000 genes. By combining short time p

SUBMITTER: Liu H 

PROVIDER: S-EPMC12581951 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature