Cupin-Type Dimethylsulfoniopropionate Lyase from Pelagibacter ubique (DddK_Pu) Catalyzes Aza-Michael Addition of Primary and Secondary Amines to Acrylic Acid.

ABSTRACT: The formation of carbon─nitrogen (C─N) bonds is a cornerstone of organic synthesis, underpinning the production of amines, imines, and nitriles found in numerous active ingredients. Among the methods for C─N bond formation, the aza-Michael addition stands out as a powerful and versatile approach. Herein, we present a biocatalytic strategy for the efficient aza-Michael addition of primary and secondary amines to acrylic acid, i.e., aza-Michaelase activity, leveraging the promiscuity of dimethylsulfoniopropionate (DMSP) lyase from Pelagibacter ubique HTCC1062 (DddK_Pu). In vivo DddK_Pu catalyzes the β-elimination of DMSP to sodium acrylate and dimethylsulfide (i.e., a retro sulfa-Michael reaction). Here, we screened DddK_Pu against a diverse library of 30 primar

SUBMITTER: Arceri D 

PROVIDER: S-EPMC12582016 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature