The Zuo1 C-terminal domain stabilizes DNA guanosine quadruplex (G4) structures located on Chromosome IX in Saccharomyces cerevisiae.

ABSTRACT: Deoxyguanosine quadruplexes (G4s) form stable non-B-DNA structures that can affect transcription, replication, and genome stability. Depending on various factors including cation binding, G4s can fold into different topologies, which can be linked to distinct function. In cells, G4 folding, function, and unfolding is affected by proteins that specifically target G4s. Zuo1 is a G4-binding protein in yeast. To investigate Zuo1 binding and its consequences on G4 formation and topology, we characterized Zuo1's interaction with G4s, both in vitro and in vivo. The C-terminus (Zuo1348-433) of Zuo1 interacts with the G4s. We characterized this interaction by combining nuclear magnetic resonance spectroscopy, single-molecule Förster Resonance Energy Transfer (smFRET), and in vivo experiments with G

SUBMITTER: Burkhart I 

PROVIDER: S-EPMC12582019 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature