Colistin-induced transition from non-mucoid to mucoid phenotype in resistant <i>Pseudomonas aeruginosa</i> ST3351.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic pathogen frequently infecting the lower airways of individuals with cystic fibrosis (iwCF). We investigated colistin (CST) resistance mechanisms in P. aeruginosa ST3351 (Psa-ST3351) and their impact on bacterial virulence. Psa-ST3351, a novel CST-resistant sequence type, was isolated from a child with CF. We demonstrate that CST resistance in Psa-ST3351 depends on the addition of 4-amino-4-deoxy-L-arabinose to lipid A. Moreover, exposure to CST induced a non-mucoid-to-mucoid phenotypic switch characterized by enhanced swarming motility, decreased susceptibility to aminoglycosides, impaired ExoS secretion via the type-III secretion system, and increased bacterial competition, all hallmarks of a chronic infection p

SUBMITTER: Rosales-Reyes R 

PROVIDER: S-EPMC12582109 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature