Nickel Binding to the c-Src SH3 Domain Facilitates Crystallization.

ABSTRACT:

Introduction

Numerous X-ray crystal structures of the c-Src SH3 domain have provided a large sampling of atomic-level information for this important signaling domain. Multiple crystal forms have been reported, with variable crystal lattice contacts and chemical crystallization conditions.

Materials and methods

We crystallized the c-Src SH3 domain in a crystallization buffer containing NiCl₂.

Results

A unique crystal structure of the Src SH3 domain in the trigonal space group H32 is determined to 1.45 Å resolution. Crystal packing and anomalous scattering reveal that this crystal form is mediated by two ordered nickel ions provided by the crystallization buffer. Nickel coordination occurs in a 2:2 stoichiometry, which dimerizes two SH3 domain monomers across

SUBMITTER: Calicdan X 

PROVIDER: S-EPMC12582252 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature