The miRNA Expression of Urinary Extracellular Vesicles in Patients With Gitelman Syndrome: The Role of hsa-let-7d-3p.

ABSTRACT: Gitelman syndrome (GS) is caused by an inactivating mutation in the SLC12A3, encoding the thiazide-sensitive sodium chloride cotransporter (NCC), leading to salt wasting and electrolyte imbalance. Our aim is to identify microRNA (miRNA) expression and explore their role from urinary extracellular vesicles (uEVs) in GS patients. In this study, both uEVs from 23 genetically confirmed GS patients and renal biopsied tissues from another 3 GS patients were extracted for small RNA sequencing. Small RNA sequencing identified 358 miRNAs from uEVs and 652 miRNAs from renal biopsied tissues. Among differentially expressed miRNAs, 20 were upregulated and 23 downregulated in uEVs, while renal biopsied tissues showed 30 upregulated and 23 downregulated miRNAs. Four miRNAs (hsa-let-7d-3p, hsa-miR-362-5p

SUBMITTER: Chen CT 

PROVIDER: S-EPMC12582352 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature