IntegrAlign: a comprehensive tool for multi-immunofluorescence panel integration through image alignment.

ABSTRACT:

Motivation

Tyramide-based multiplex-immunofluorescence (mIF) enables the simultaneous analysis of up to seven protein markers on a whole slide, providing a comprehensive approach to study the tumor microenvironment. Integrating multiple mIF panels through image alignment of serial slide significantly expands the number of cell populations analysed in a single space. IntegrAlign was developed to optimize this integration on serial whole slides, enhancing the value and applicability of -mIF for comprehensive spatial analyses and enabling biomarker discovery at scale.

Results

IntegrAlign, leveraging the SimpleITK toolkit, applies a two-step alignment using rigid and B-spline transformations to integrate serial mIF whole slides. Validation on simulated and real datasets demonstr

SUBMITTER: Hermet L 

PROVIDER: S-EPMC12582367 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature