Deciphering estrogen receptor alpha-driven transcription in human endometrial stromal cells via transcriptome, cistrome, and integration with chromatin landscape.

ABSTRACT:

Objective

To investigate estrogen receptor gene 1 (ESR1) and estrogen-driven transcription in human endometrial stromal cells.

Design

RNA sequencing (RNA-seq) and Cleavage Under Targets and Release Using Nuclease (Cut&Run) were performed on telomerase-immortalized human endometrial stromal cells with Clustered Regularly Interspaced Short Palindromic Repeats-mediated ESR1 activation. Hi-C-based chromatin architecture analysis (H3K27ac HiChIP) was conducted in primary endometrial stromal cells.

Subjects

Biopsies from two healthy, reproductive-aged volunteers with regular menstrual cycles and no history of gynecological malignancies.

Exposure

The ESR1-activated and control endometrial stromal cells were treated with estradiol (E2) or vehicle. Primary endometrial s

SUBMITTER: Montague Redecke SG 

PROVIDER: S-EPMC12582374 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature