A Fragmentation behavior-guided UHPLC-Q-Orbitrap HRMS method for the quantitative analysis of 26 perfluoroalkyl substances and their alternatives in water.

ABSTRACT: Perfluoroalkyl substances (PFASs), including newly introduced alternatives, are of global concern due to their environmental persistence, bioaccumulation, and potential health risks. We developed and validated a sensitive, selective UHPLC-Q-Orbitrap HRMS method for the comprehensive analysis of 26 PFASs-including perfluorocarboxylic acids (PFCAs), perfluorosulfonic acids (PFSAs), and emerging substitutes-in diverse water matrices. Sample preparation used WAX solid-phase extraction with isotope-labeled internal standards and separation on a BEH C18 column; quantitative performance was assessed under Full MS, targeted SIM, and PRM. The method achieved excellent linearity, sub-ng/L detection capability, and robust recoveries and precision across matrices, with PRM offering the best balance of

SUBMITTER: Shao G 

PROVIDER: S-EPMC12582490 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature