Novel pyrimidine-substituted chalcones: In vitro antioxidant properties and cytotoxic effects against human cancer cell lines.

ABSTRACT: A series of novel pyrimidine-substituted chalcones were synthesized, purified, and characterized. Four selected compounds (CFMPY 4, 15, 17, and 28) exhibited modest antioxidant activity across six In vitro radical scavenging assays. The In vitro cytotoxic (anticancer) potential of five synthesized chalcones (CFMPY-2, 4, 15, 17, and 28) was evaluated using MTT, Sulforhodamine B (SRB), DNA fragmentation, Nuclear staining, and Farnesyl transferase assays. Notably, this study is the first to employ a DNA fragmentation assay to assess the anticancer activity of these test compounds. MTT and SRB assays revealed significant cytotoxicity in HeLa and A549 cell lines for all compounds except CFMPY 17, with IC50 values ranging from 2.28 to 5.48 µg/ml, demonstrating comparable or superior efficacy to

SUBMITTER: Nayak P 

PROVIDER: S-EPMC12582495 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature