Elastic analysis bridges structure and dynamics of an AAA+ molecular motor.

ABSTRACT: Proteins carry out cellular functions by changing their structure among a few conformations, each characterised by a different energy level. Therefore, structural changes, energy transformations, and protein function are intimately related. Despite its central importance, this relationship remains elusive. For example, while many hexameric ATPase motors are known to function using a hand-over-hand alternation of subunits, how energy transduction throughout the assembly's structure drives the hand-over-hand mechanism is not known. In this work, we unravel the energetic basis of hand-over-hand in a model AAA+ motor, RuvB. To do so, we develop a general method to compute the residue-scale elastic pseudoenergy due to structure changes and apply it to RuvB structures, recently resolved through

SUBMITTER: Mello VH 

PROVIDER: S-EPMC12582506 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature