Clathrin-mediated endocytosis regulates root endodermal suberization via ROS.

ABSTRACT: Endomembrane trafficking (ET) plays a crucial role in plant adaptation to environmental stresses, yet its involvement in endodermal root suberization remains poorly understood. Here, we show that disruption of clathrin-mediated endocytosis (CME) or canonical exocytosis led to an ectopic suberin deposition in the Arabidopsis root endodermis toward the root tip. Genetic disruption of endocytosis phenocopied the effects of the CME inhibitor ES9-17, while genetic disruption of clathrin-independent endocytosis led to reduced suberization, suggesting distinct, pathway-specific roles in regulating suberin deposition. Ectopic suberization upon CME inhibition required the CIFs-SGN3-SGN1-RBOHF/D signaling axis, independent of abscisic acid. Notably, CME disruption led to the accumulation of RBOHF in

SUBMITTER: Martinez Pacheco J 

PROVIDER: S-EPMC12582552 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature