Anti-fibrotic effect of human amniotic fluid stem cells in biliary epithelial-mesenchymal transition of liver ductal organoid.

ABSTRACT:

Purpose

In biliary atresia (BA), it has been demonstrated that biliary epithelial-mesenchymal transition (EMT) of reactive ductular cells is associated with liver fibrosis. This study aimed to develop an ex vivo biliary EMT model of liver ductal organoids for exploring the involvement of biliary EMT in fibrogenesis and to investigate whether human amniotic fluid stem cells (hAFSCs) can mitigate the biliary EMT process.

Methods

Liver ductal organoids were generated from the intrahepatic bile duct of healthy neonatal mice. Biliary EMT was induced in organoids by the administration of transforming growth factor beta-1 (TGF-β1) in culture medium. hAFSCs were co-cultured with organoids during biliary EMT induction. Expression of biliary epithelial cells, mesenchymal cells, myofib

SUBMITTER: Chusilp S 

PROVIDER: S-EPMC12582591 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature