Boosting the cellulolytic oxidative and hydrolytic enzyme systems in Myceliophthora thermophila for the efficient enzymatic saccharification of lignocellulosic biomass.

ABSTRACT: The thermophilic fungus Myceliophthora thermophila serves as a vital platform for producing cellulolytic complex enzymes. However, their efficiency still requires enhancement to meet the cost-effective demands of lignocellulosic biomass conversion. Herein, secretome analysis revealed that the cellulolytic enzyme system of M. thermophila comprises the oxidative system consisting of lytic polysaccharide monooxygenases (LPMOs) and the hydrolytic system that includes endoglucanase, cellobiohydrolase, and β-glucosidase. Both in vitro supplementation and in vivo overexpression of MtLPMOs with C1 or C1/C4 oxidizing activity enhanced the enzymatic saccharification of Avicel using M. thermophila fermentation broth, resulting in a maximum increase of 485% in oxidized cello-oligosaccharides productio

SUBMITTER: Bai R 

PROVIDER: S-EPMC12583286 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature