Quantification of small (1-10 µm) microplastic particles in soil matrices using automated scanning electron microscopy: possibilities and limitations.

ABSTRACT: Scanning electron microscopy (SEM) provides superb resolution power and allows distinguishing microplastic particles (MPs) from other particles based on elemental ratios derived from energy dispersive x-ray (EDX) analysis. Polycarbonate (PC) membranes are typically used to isolate MPs from suspensions for SEM investigations. This, however, results in poor (atomic weight) contrast between MPs and the underlying PC membrane, challenging automated particle detection. To introduce an elemental contrast between MPs and the PC membrane, a gold (Au) coating was applied to the PC membrane before use. Monte Carlo simulations conducted to optimize operational conditions of the SEM suggest an acceleration voltage of 3 kV in combination with a 40 nm Au coating of PC membranes as most appropriate. Stoc

SUBMITTER: Kaegi R 

PROVIDER: S-EPMC12583325 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature