Differential effects of KRAS G-domain and hypervariable region mutants on cancer phenotypes.

ABSTRACT: Identification of tumor driver genes in cancer has led to the discovery of specific allelic variants that are useful as predictive or prognostic markers. In colorectal cancer (CRC), the K-Ras GTPase is one of the most mutated oncoproteins; therefore, understanding the functional consequences of its mutations will allow us to exploit allele-specific vulnerabilities and propose potential mechanisms of resistance in cancer therapeutics. In this study, cellular phenotypic consequences of the K-Ras mutants K117I, K117N, A146T, E168K, and K172T identified in Filipino young-onset CRC (YO-CRC) patients were assessed in HCT116, Caco-2, and NIH3T3 cells. In assays assessing DNA doubling in 2D cultures and viability in 3D multicellular spheroids, only K117I, K117N, and A146T exhibited enhanced prolif

SUBMITTER: de Borja JAD 

PROVIDER: S-EPMC12583673 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature