Real-Time In Vivo Monitoring of Cholinergic Neurotransmission in the Mouse Brain Using a Microelectrochemical Choline Biosensor.

ABSTRACT: The measurement of choline as a biomarker for in vivo cholinergic neurotransmission is a valuable tool in the study of a range of CNS pathologies. However, the continuous detection of cholinergic neurotransmission in selective brain regions in the mouse brain remains challenging and underexploited. Here, we have refined an established choline oxidase (ChOx) microelectrochemical biosensor and validated its use for long-term recording in the freely moving mouse. Using a 75-μm diameter polymer-ChOx composite disc electrode, we have successfully monitored stable and reproducible chronic real-time changes in choline-induced amperometric currents in vivo. Local infusions of choline and acetylcholine resulted in an increase in biosensor current in the hippocampus, while the inhibition of endogeno

SUBMITTER: Doyle S 

PROVIDER: S-EPMC12583884 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature