Impact of Hanks Kinase-Dependent Phosphorylation of CodY on the Physiology and Virulence in Bacillus cereus.

ABSTRACT: CodY acts as a key regulatory protein involved in adaptive responses in low-G+C Gram-positive bacteria. This global transcriptional regulator diagnoses the nutritional status of the cell and responds by regulating transcription of genes involved in metabolism, differenciation and virulence. Phosphoproteomic studies evidenced that CodY is phosphorylated on its serine 215 in Bacillus subtilis. In Bacillus cereus, CodY is also phosphorylated by the Hanks kinases PrkC and YbdM. CodY phosphorylation negatively affects its DNA-binding properties. We constructed B. cereus mutant strains where the codY wild-type allele has been replaced by codY-S215D or codY-S215A, encoding a phosphomimetic or a phosphoablative CodY derivative, respectively. We showed that the phosphomimetic mutation leads to a no

SUBMITTER: Kortebi M 

PROVIDER: S-EPMC12583987 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature