Efficient and rapid cell surface functionalization: a sub-minute selenol-yne click reaction for bioconjugation.

ABSTRACT: Cell surface engineering plays a crucial role in enhancing the functionality and therapeutic potential of living cells through precise and controllable surface modifications. However, traditional conjugation strategies often suffer from limited efficiency or inherent complexity. In this work, we developed a selenol-yne click (SYC) reaction for efficient and rapid cell surface functionalization, enabling covalent attachment of diverse payloads to a variety of cell types. The SYC reaction enables highly efficient conjugation of fluorescent molecules, micron-scale "backpack" particles, and functional polymers onto cell surfaces, including HeLa, B16-OVA, and Jurkat T cells. Drug-loaded backpacks carried by the cells demonstrated effective drug delivery to target cells. Moreover, cell surface g

SUBMITTER: Shan F 

PROVIDER: S-EPMC12584132 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature