Mechanistic insights into fungal succession mediated by Pichia kudriavzevii during aerobic deterioration of ensiled total mixed ration.

ABSTRACT:

Background

Aerobic deterioration of ensiled total mixed ration (ETMR) represents a critical challenge in livestock feed preservation, yet the microbial drivers of this process remain poorly characterized. Although Pichia kudriavzevii has been identified as the predominant spoilage yeast in ETMR, its ecological role in fungal community assembly and succession during aerobic deterioration remains unclear. This study elucidates the impact of P. kudriavzevii on aerobic stability and fungal succession in alfalfa and oat based ETMR, inoculated with or without P. kudriavzevii and analyzed after 42 days ensiling.

Results

Chemical analyses and high-throughput sequencing demonstrated that P. kudriavzevii inoculation significantly reduced aerobic stability (P<0.05) without affecting fe

SUBMITTER: Jiang D 

PROVIDER: S-EPMC12584327 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature