<i>In situ</i> Raman spectroscopic relative quantitative analysis of sulfur metabolic dynamics in deep-sea microorganisms.

ABSTRACT: Deciphering dynamic regulation of microbial sulfur metabolism in deep-sea environments is critical for understanding global biogeochemical cycles and climate feedback mechanisms. Current analytical approaches face limitations in achieving quantitative, in situ visualization of microbial metabolic processes, including susceptibility to environmental interference during sampling and analysis, leading to impaired data accuracy. This study developed an innovative method based on confocal Raman spectroscopy utilizing nitrogen as an internal standard for metabolite quantification. Taking sulfate, which is a major component of seawater and essential for the sulfur cycle, as a model, we quantified it in solid medium and monitored the in situ metabolic processes of deep-sea Erythro

SUBMITTER: He W 

PROVIDER: S-EPMC12584613 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature