Development and evaluation of multiepitope fusion proteins for serological diagnosis of animal brucellosis.

ABSTRACT: Brucellosis, a zoonotic disease caused by Brucella spp., leads to severe reproductive issues in livestock and economic losses. Current serological diagnostics using lipopolysaccharide (LPS) antigens often exhibit cross-reactivity, reducing diagnostic specificity. This study aimed to develop multiepitope fusion proteins based on Brucella B-cell epitopes using bioinformatics tools and the Immune Epitope Database (IEDB) to enhance diagnostic accuracy. B-cell epitopes from major Brucella outer membrane proteins and other antigenic proteins were predicted using bioinformatics tools (BepiPred, ABCpred, and IEDB). Two fusion proteins were designed and produced. The diagnostic performance of the two fusion proteins was evaluated using indirect enzyme-linked immunosorbent assay

SUBMITTER: Guo L 

PROVIDER: S-EPMC12584648 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature