Proteomic comparison of epidemic Australian <i>Bordetella pertussis</i> biofilm cells.

ABSTRACT: Bordetella pertussis causes whooping cough, a severe respiratory infectious disease. Studies have compared the currently dominant single nucleotide polymorphism (SNP) cluster I (pertussis toxin promoter allele, ptxP3) and previously dominant SNP cluster II (ptxP1) strains as planktonic cells. Since biofilm formation is linked with B. pertussis pathogenesis in vivo, this study compared the biofilm formation capabilities of representative strains of cluster I and cluster II. Confocal laser scanning microscopy found that the cluster I strain had a denser biofilm structure compared to the cluster II strain. Differences in protein abundance of the biofilm cells were then compared using tandem mass tagging and high-resolution multiple reaction monitoring. In to

SUBMITTER: Suyama H 

PROVIDER: S-EPMC12584666 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature