A specific, stable, and accessible LAMP assay targeting the HSP70 gene of <i>Trypanosoma cruzi</i>.

ABSTRACT: Diagnostic delays prevent most Chagas disease patients from receiving timely therapy during the acute phase when treatment is effective. Quantitative PCR (qPCR)-based diagnostic methods provide high sensitivity during this phase but require specialized equipment and complex protocols. More simple and cost-effective tools are urgently needed to expand early Chagas disease diagnosis in low-income endemic regions. Here, we present a loop-mediated isothermal amplification (LAMP) that targets a highly conserved region in the HSP70 gene of Trypanosoma cruzi, the causative agent of Chagas disease. This assay demonstrates species-specific amplification across multiple parasite genetic lineages while maintaining stability after 1 hour of incubation and at least 8 months of storage at -20°C.

SUBMITTER: Gutierrez Guarnizo SA 

PROVIDER: S-EPMC12584694 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature