Rapid direct disk diffusion testing for antibiotic resistance in urinary tract infections: a bacterial concentration-adjusted approach.

ABSTRACT: Urinary tract infections are among the most prevalent bacterial infections worldwide, typically diagnosed using clinical symptoms, dipstick tests, and laboratory methods requiring standardized bacterial suspensions for antibiotic susceptibility testing (AST). This study evaluated two rapid disk diffusion methods for urine samples that incorporate bacterial concentration into the analysis, eliminating the need for standardized suspensions. The threshold-adapted approach compares inhibition zones to concentration-specific breakpoints derived from reference strains, while the regression-based method transforms inhibition zones at various concentrations into predicted standard (0.5 McFarland) values using a linear model. In both methods, urine and antibiotic disks are applied to one agar plate

SUBMITTER: Sabersky-Mussigbrodt H 

PROVIDER: S-EPMC12584718 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature