Establishment of an RAA-CRISPR/Cas12a assay based on <i>CpSge1</i> for rapid detection of <i>Cryphonectria parasitica</i>.

ABSTRACT: Chestnut blight disease caused by Cryphonectria parasitica is a serious branch disease that occurs worldwide, especially in Europe and North America. In recent years, chestnut blight disease has also been severe and even posed a great threat to the healthy development of chestnut orchards in some areas of China. Accurate and rapid detection of C. parasitica during the initial stages of the disease is helpful to take corresponding prevention and control measures in advance. In this study, we selected the CpSge1 (Gti1/Pac2 transcription factor family) of C. parasitica as the detection target and established a rapid and visual detection system of C. parasitica that combined the recombinase-aided amplification (RAA) and CRISPR/Cas12a, called CpSge1-RAA

SUBMITTER: Wu H 

PROVIDER: S-EPMC12584721 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature