Isolation and characterization of strictly lytic bacteriophages against carbapenem-resistant <i>Enterobacter cloacae</i> complex.

ABSTRACT: The global surge of carbapenem-resistant Enterobacter cloacae complex (CR-ECC) poses a significant clinical challenge due to limited treatment options. This study aimed to isolate and characterize lytic bacteriophages (phages) targeting CR-ECC. CR-ECC CYEBC080 was used as the bacterial host for isolating lytic phages, and a comprehensive evaluation was conducted on isolated phages, including phage stability under various pH and temperature conditions, host range analysis, killing curves, and therapeutic efficacy in Galleria mellonella larvae and a murine bacteremia model. Twelve lytic phages with distinct random amplified polymorphic DNA patterns were isolated, and transmission electron microscopy confirmed their classification under the Straboviridae family within the

SUBMITTER: Kuo H-Y 

PROVIDER: S-EPMC12584729 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature