Bioluminescent reporter influenza A viruses to track viral infections.

ABSTRACT: Influenza A viruses (IAVs) infect a wide range of mammalian and bird species and are responsible for seasonal outbreaks and occasional pandemics of great consequences in humans. Studying IAVs requires methods to detect the presence of the virus in infected cells or animal models. Recombinant IAV-expressing fluorescent proteins has allowed monitoring of viral infection in cultured cells and ex vivo in the organs of infected animals. However, fluorescent-expressing IAVs are often attenuated and are not suited for the imaging of infected animals using in vivo imaging systems (IVISs). To overcome this limitation, we generated a recombinant pandemic influenza A/California/04/2009 H1N1 (pH1N1)-expressing nanoluciferase (Nluc) from the non-structural viral segment, hereafter referre

SUBMITTER: Barre RS 

PROVIDER: S-EPMC12584733 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature