Building a human pluripotent stem cell-based gonadal niche: improving in vitro systems with in vivo insights.

ABSTRACT:

Background

The gonadal somatic niche is crucial for sex determination and gamete formation throughout the human life cycle. However, key steps in gonadal somatic lineage differentiation occur during embryonic and foetal development, making them difficult to study in humans. In vitro differentiation models are therefore needed to investigate gonadal development, support in vitro gametogenesis, and study infertility. A comprehensive overview of gonadal somatic niche differentiation, both in vivo and in vitro, is thus crucial.

Objective and rationale

This review connects in vivo knowledge with in vitro differentiation systems for gonadal somatic niches, predominantly focusing on cell-cell signalling factors. It evaluates existing in vitro protocols for differentiating testicula

SUBMITTER: Lakshmipathi M 

PROVIDER: S-EPMC12584898 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature