Identification of the <i>Yarrowia lipolytica</i> cysteine sulfinic acid decarboxylase gene using a newly developed method with optimized <i>Escherichia coli</i> combinations of mutant alleles.

ABSTRACT: To develop a low-cost, environmentally friendly taurine fermentation method for sustainable marine fish culture using feed derived from photosynthetically produced agricultural products, it is crucial to study cysteine sulfinic acid decarboxylase (CSAD), a key enzyme in the taurine biosynthetic pathway in applicable microorganisms. In this study, a method was devised to screen for CSAD genes using Escherichia coli growth as an indicator, based on sulfur assimilation following the decarboxylation of l-cysteic acid, a taurine precursor compound. The E. coli used has a double deletion mutation of cysA (sulfate/thiosulfate ABC transporter) and ssuD (FMNH₂-dependent alkanesulfonate monooxygenase) genes. If needed, an additional defect in enzyme genes, such

SUBMITTER: Nishikawa M 

PROVIDER: S-EPMC12585060 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature