Effect of dopamine on TGF-β2 secretion by human retinal pigment epithelial cells and the underlying mechanism.

ABSTRACT:

Background

Dopamine is known to play a role in the development of myopia. In this study, we investigated the effect of dopamine (DA) on the secretion of transforming growth factor-β2 (TGF-β2) in human retinal pigment epithelial (RPE) cells. Additionally, we examined the effects of SCH23390 (D1 receptor antagonist) and sulpiride (D2 receptor antagonist) on TGF-β2 levels in ARPE-19 cells to elucidate the underlying mechanism of DA in myopia development.

Methods

Human RPE cells were cultured with or without different concentrations of DA, SCH23390, or sulpiride. Cell proliferation was examined using the CCK-8 assay. Cell migration was assessed by performing a cell scratch (wound healing) assay. The expression of TGF-β2 was evaluated at the mRNA and protein levels by real-time P

SUBMITTER: Fan J 

PROVIDER: S-EPMC12585080 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature