Loss of Insulin-Positive Cell Clusters Precedes the Decrease in Islet Frequency and β-Cell Area in Type 1 Diabetes.

ABSTRACT: In type 1 diabetes (T1D), insulin (INS) deficiency results from immune-mediated destruction of β-cells. The majority of functional β-cell mass is typically lost within months to years of disease diagnosis, but the timing and nature of this loss, particularly in early disease stages, remain unclear. We developed a whole-slide scanned image analysis pipeline for semiautomated quantitation of endocrine area, islet frequency, interislet distance, and endocrine object size distribution in 145 human pancreata from 60 donors without diabetes, 19 donors with single autoantibody positivity, 10 with multiple autoantibody positivity (mAAb+), 16 with recent-onset T1D (duration 0-1 year), 23 with medium-duration T1D (1-7 years), and 17 with long-duration T1D (≥7 years). We observed age-related differen

SUBMITTER: Drotar DM 

PROVIDER: S-EPMC12585168 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature