Development of a ferroptosis-related signature and identification of NOTCH2 as a novel prognostic biomarker in pancreatic cancer.

ABSTRACT:

Background

Ferroptosis, a regulated form of iron-dependent cell death, has shown promise as an anti-tumor mechanism. However, its role in pancreatic cancer remains largely unexplored. This study aimed to identify a ferroptosis-related prognostic signature and key biomarkers.

Methods

Transcriptomic profiles and clinical data of pancreatic cancer patients were obtained from the GEO and TCGA databases. A prognostic signature was constructed using LASSO and Cox regression analysis. The role of a key gene, NOTCH2, was investigated through somatic mutation, functional enrichment, immune infiltration, and drug sensitivity analysis. In vitro, the expression of NOTCH2 was confirmed by Western blot, and its effects on cell proliferation and migration were assessed using MTT, co

SUBMITTER: Zhang S 

PROVIDER: S-EPMC12585956 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature