Stable coexistence and phenol-degradation expression of Cupriavidus sp. strain P-10 and Comamonas thiooxydans strain R2 in phenol-competitive chemostat culture.

ABSTRACT: We investigated the mechanism underlying the coexistence of the phenol-degrading bacteria Cupriavidus sp. strain P-10 and Comamonas thiooxydans strain R2 using a chemostat culture supplemented with phenol as the sole carbon and energy source. Quantitative PCR (qPCR) targeting the genes encoding the large subunit of phenol hydroxylase dmpN and aphN for strains P-10 and R2, respectively, revealed the coexistence of the two strains even under phenol-limiting conditions. According to the reverse transcription qPCR targeting dmpN, aphN, catechol 2,3-dioxygenase genes (dmpB and aphB), and catechol 1,2-dioxygenase gene (catA) of strain P-10, both strains transcribed these genes, suggesting that phenol was metabolized individually. However, mathematical simulations demonstrated that the two strain

SUBMITTER: Suzuki K 

PROVIDER: S-EPMC12586219 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature