Optimizing post brain death vascularized composite graft quality through ex vivo restoration of S-nitrosothiol homeostasis.

ABSTRACT: A challenge in vascularized composite allotransplantation (VCA) is mitigating tissue damage within the composite secondary to brain death (BD). Loss of central nervous system function disrupts S-nitrosothiol (SNO) homeostasis to produce systemic hypoxia and ischemic injury during the donor support phase. We reasoned that addition of an S-nitrosylating agent to the preservation solution could correct this damage during ex vivo storage. Employing a swine BD preparation, we excised VC tissues (abdominal blocks and limbs) after a 16-h period of systemic support. The composites were perfused with/without the S-nitrosylating agent ethyl nitrite (ENO) present in the preservation solution. Flow rates and resistance were recorded during the storage period; tissue hypoxia was also quantified. BD dec

SUBMITTER: Zhu L 

PROVIDER: S-EPMC12586435 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature