Towards site-specific information on PET degrading enzymes using NMR near operational temperature.

ABSTRACT: PETases are enzymes that can break down the poly-ethylene terephthalate (PET) polymer in its constituent building blocks. This enzymatic recycling process offers a sustainable solution for producing new, high-quality plastics from previously used materials. NMR spectroscopy can help in understanding and ultimately improving these enzymes but is always confronted with the lengthy step of acquisition and interpretation of triple resonance spectra for the spectral assignment. Here, we explore whether this step can be made more efficient by recording the spectra directly at high temperature, which simultaneously corresponds to more realistic working conditions for the enzyme. Taking the inactive variant of LCC^ICCG as an example, we compare spectral quality at 30°C and 50°C, and find

SUBMITTER: Gabrielli V 

PROVIDER: S-EPMC12586641 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature