Developing and optimizing a biocompatible tauopathy model using extracellular vesicle-mediated gene delivery.

ABSTRACT:

Introduction

Tauopathy models are essential in vitro systems for investigating tau-targeted therapies and advancing Alzheimer's disease research. Extracellular vesicles (EVs), owing to their high biocompatibility, low toxicity, and reduced immunogenicity, represent promising carriers for gene delivery and disease modeling.

Methods

We investigated the potential of EVs as a delivery system for the human four-repeat tau isoform lacking N-terminal sequences (4R0N) and enhanced green fluorescent protein (EGFP) into Neuro-2a cells. EV-mediated transfection efficiency was compared with conventional methods, including lentiviral and chemical (lipofectamine and polyethyleneimine, PEI) approaches. Response surface methodology (RSM) was used to optimize EV-mediated delivery parameters.

SUBMITTER: Ghadami S 

PROVIDER: S-EPMC12587183 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature