Analysis of the main components of LSPN based on broadly targeted metabolomics technology.

ABSTRACT:

Introduction

This study aimed to (1) establish a broadly targeted metabolomics workflow to evaluate batch-to-batch variability in Panax notoginseng-derived Panax notoginseng stem/leaf-derived oral liquid (LSPN) preparations, (2) identify critical process-induced metabolite differences (e.g., saponin degradation products), and (3) correlate these differences with pharmacopoeial quality markers (e.g., Rg1/Rb1 ratios).

Methods

Our hypothesis was that thermal extraction parameters would significantly alter saponin profiles, detectable via orthogonal metabolomic approaches (HPLC-MS/MS, PCA).

Results

The results showed a strong positive correlation (pearson correlation coefficient >0.92) between triterpenoid saponins in LSPN-S and LSPN-C. Thirty-eight triterpe

SUBMITTER: Qin F 

PROVIDER: S-EPMC12587676 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature